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LDI-G2-5 Laser Diode Illuminator

LDI-G2-5

The LDI-G2-5 builds on the G2 platform with expanded power and spectral reach. Alongside the core visible lines, it extends into the near-infrared with a 730 nm channel, ideal for deep-tissue imaging and multiplexing imaging techniques. Combining brighter illumination with enhanced stability, the LDI-G2-5 delivers confidence in results across both traditional and cutting-edge applications. As with the 4-line model, optional 488 nm and 577 nm lasers can be included for GFP and mCherry imaging. Note that we can also work with you to provide solutions for your unique needs.

Key features

  • 5 laser lines from visible to near-infrared (up to 730 nm)
  • Up to 1000 mW output power per line
  • Feedback-controlled optical stability
  • Optional 488 nm & 577 nm lasers
  • No user alignment required
  • TTL & Analog control
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References and product variations

Part number

Combined wavelengths (nm)

Options

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Part number LDI-G2-5

405 / 470 / 555 / 640 / 730

Part number LDI-G2-5-577

405 / 470 / 577 / 640 / 730

Part number LDI-G2-5-488

405 / 488 / 555 / 640 / 730

Part number LDI-G2-5-488-577

405 / 488 / 577 / 640 / 730

Performance by Wavelength

Wavelength

Optical Power Min (1)

CW Stability (typ.) (2)

Max Rise Time (typ.) (3)

Max On/Off Frequency (4)

405

Optical Power Min (1) 450 mW

CW Stability (typ.) (2) < 0.2%

Max Rise Time (typ.) (3) < 5 µs

Max On/Off Frequency (4) > 1000 Hz

470 or 488

Optical Power Min (1) 1000 mW (5)

CW Stability (typ.) (2) < 0.2%

Max Rise Time (typ.) (3) < 5 µs

Max On/Off Frequency (4) > 1000 Hz

555 or 577

Optical Power Min (1) 700 or 500 mW (6)

CW Stability (typ.) (2) < 0.2%

Max Rise Time (typ.) (3) < 2 ms

Max On/Off Frequency (4) 100 Hz

640

Optical Power Min (1) 1000 mW

CW Stability (typ.) (2) < 0.2%

Max Rise Time (typ.) (3) < 5 µs

Max On/Off Frequency (4) > 1000 Hz

730

Optical Power Min (1) 750 mW

CW Stability (typ.) (2) < 0.2%

Max Rise Time (typ.) (3) < 5 µs

Max On/Off Frequency (4) > 1000 Hz

(1) Defined in accordance with the QUAREP-LiMi WG 1 standard, “Illumination Power, Stability, and Linearity Measurements for Confocal and Widefield Microscopes V.2”, Section 8, with power output measured at the fiber output.

(2) Measured at 100% intensity, 23°C ± 2°C.

(3) Defined in accordance with the QUAREP-LiMi WG 1 standard, “Illumination Power, Stability, and Linearity Measurements for Confocal and Widefield Microscopes V.2”, Section 8, with power output measured at the fiber output.

(4) Measured at 100% intensity, 50% duty cycle

(5) 470 or 488 - Optical Power Min: 488 nm optical power min preliminary estimate. Actual output power pending product release.

(6) 555 or 577 - Optical Power Min: 577 nm optical power min preliminary estimate. Actual output power pending product release.

Optical characteristics

Output Power

Up to 1000 mW per line
+ higher power density via 400 µm single
fiber

Optical Stability

Active feedback control
< 0.2% CW stability (10× improvement)

Laser Lines

5 lines: DAPI | FITC/GFP |
TRITC/mCherry | Cy5 | 730 nm NIR
+ optional 488 nm & 577 nm

NIR Channel (730 nm)

✔  Deep-tissue imaging
Multiplexing applications

Beam Quality

Improved beam quality
Cleaner, more stable output profile

Fiber Output

400 µm single  →  higher power density

User Alignment

Not required

LDI-G2-5 Laser Diode Illuminator General specifications

Output Options

Optical fiber
(400 µm single, 0.39 NA)

Control Options

TTL (> 2.3 V) | Analog (0–5 V)
USB–DSP (virtual COM port)

Intensity Control

0.1% steps (10× finer)
Full linear range

Safety

Interlocked housing | Key interlock
IEC 60825 compliant

QUAREP-LiMi WG1

✔ Compliant

Dimensions

318 × 234 × 146 mm
(12.5" × 9.2" × 5.75")

Weight

~ 4 kg (~9 lbs)

Operating Temp.

15 to 30 °C

Storage Temp.

-18 to 50 °C

Humidity

< 80% non-condensing

Voltage

90–220 V AC, 50–60 Hz

Warranty

2 years

Available options

Fiber sets

LDI Breakout Box

Fiber Bend Box

External Despeckler

Applications / Techniques

Spinning Disk Microscopy

Widefield Microscopy

Light Sheet Microscopy

Structured Illumination Microscopy (SIM)

Super Resolution Imaging

TIRF

PALM / STORM / DNA-PAINT

FRAP

Photoactivation / Photoconversion

Photoablation

Optogenetics

Near Infrared (NIR) Imaging — 730 nm

Neuroscience / Physiology

Cellular Biology

Single Molecule FISH (smFISH)

Spatial Transcriptomics / Proteomics

Digital Pathology