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LDI-4 Laser Diode Illuminator

LDI-4

The LDI-4 laser diode illuminator was designed in direct response to researcher demand for a streamlined, 4-channel solution covering the most essential fluorescence probes: DAPI (405 nm), FITC/GFP (470 or 488 nm), TRITC/mCherry (555 or 577 nm) and Cy5 (640 nm). Built on the same robust platform as the LDI-7, it delivers up to 1000 mW per line with feedback-controlled optical stability, TTL and analog modulation, and zero user alignment — making it an ideal drop-in laser source for spinning disk confocal, widefield, TIRF and super-resolution setups.

You can also check out our new generation of LDI-4: LDI-G2-4 Laser Diode Illuminator

Key features

  • 4 laser lines covering the most widely used fluorescence probes
  • Up to 1000 mW output power per line
  • Feedback-controlled optical stability
  • Optional 488 nm & 577 nm lasers
  • No user alignment required
  • TTL & Analog control
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References and product variations

Part number

Combined wavelengths (nm)

Options

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Part number LDI-4

405 / 470 / 555 / 640

Part number LDI-4-577

405 / 470 / 577 / 640

Part number LDI-4-488

405 / 488 / 555 / 640

Part number LDI-4-488-577

405 / 488 / 577 / 640

Performance by Wavelength

Wavelength

Optical Power Min (1)

CW Stability (typ.) (2)

Max Rise Time (typ.) (3)

Max On/Off Frequency (4)

405

Optical Power Min (1) 300 mW

CW Stability (typ.) (2) < 2 %

Max Rise Time (typ.) (3) < 10 µs

Max On/Off Frequency (4) > 1000 Hz

470 or 488

Optical Power Min (1) 1000 mW

CW Stability (typ.) (2) < 2%

Max Rise Time (typ.) (3) < 10 µs

Max On/Off Frequency (4) > 1000 Hz

555 or 577

Optical Power Min (1) 1000 or 700 mW

CW Stability (typ.) (2) < 2%

Max Rise Time (typ.) (3) < 2 ms

Max On/Off Frequency (4) 100 Hz

640

Optical Power Min (1) 400 mW

CW Stability (typ.) (2) < 2%

Max Rise Time (typ.) (3) < 10 µs

Max On/Off Frequency (4) > 1000 Hz

(1) Optical power measured at 100% intensity, 23°C ± 2°C.

(2) Continuous Wave Stability: Standard Deviation relative to mean power, at 100% intensity, 23°C ± 2°C.

(3) Optical power measured at 100% intensity, 23°C ± 2°C.

(4) Measured at 100% intensity, 50% duty cycle

Optical characteristics

Output Power

Up to 1000 mW per line

Optical Stability

Feedback-controlled
< 2% CW stability

Intensity Control

1% steps
100:1 linear dynamic range

Laser Lines

Up to 7 lines : 405 / 445 / 470 or 488
520 / 555 or 577 / 640 / 730 nm

NIR Channel (730 nm)

✔ Deep-tissue imaging
Extended spectral range beyond visible

Beam Quality

Standard multimode output

Fiber Output

400 µm bifurcated

User Alignment

Not required

LDI-4 Laser Diode Illuminator General specifications

Output Options

Optical fiber
(400 µm bifurcated, 0.39 NA)

Control Options

TTL (> 2.3 V) | Analog (0–5 V)
USB–DSP (virtual COM port)

Safety

Interlocked housing | Key interlock
IEC 60825 compliant

QUAREP-LiMi WG1

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Dimensions

318 × 234 × 146 mm
(12.5" × 9.2" × 5.75")

Weight

~ 4 kg (~9 lbs)

Operating Temp.

15 to 30 °C

Storage Temp.

-18 to 50 °C

Humidity

< 80% non-condensing

Voltage

90–220 V AC, 50–60 Hz

Warranty

2 years

Available options

Fiber sets

LDI Breakout Box

Fiber Bend Box

External Despeckler

Applications / Techniques

Spinning Disk Microscopy

Widefield Microscopy

Light Sheet Microscopy

Structured Illumination Microscopy (SIM)

Super Resolution Imaging

TIRF

PALM / STORM / DNA-PAINT

FRAP

Photoactivation / Photoconversion

Photoablation

Optogenetics

Neuroscience / Physiology

Cellular Biology

Single Molecule FISH (smFISH)

Spatial Transcriptomics / Proteomics

Digital Pathology