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LDI-4 Laser Diode Illuminator

LDI-4

The LDI-4 laser diode illuminator is a user inspired version of the original LDI-7. Many researchers were asking for a laser launch covering only the 4 basic lines: DAPI, FITC, TRITC, Cy5. The LDI-4 has been designed with exactly these lines in mind. While the number of lines inside the box has been reduced, no compromises have been made on quality and performance. This unit is also available with a 488 nm laser for FITC/GFP and with a 577 nm laser for TRITC/mCherry…or both! Note that we can work with you to provide solutions for your unique needs.

Key features

  • 4 laser lines covering the most widely used fluorescence probes
  • Up to 1000 mW output power per line
  • Feedback-controlled optical stability
  • Optional 488 nm & 577 nm lasers
  • No user alignment required
  • TTL & Analog control
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References and product variations

Part number

Combined wavelengths (nm)

Options

Ask for a quote

Part number LDI-4

405 / 470 / 555 / 640

Part number LDI-4-577

405 / 470 / 577 / 640

Part number LDI-4-488

405 / 488 / 555 / 640

Part number LDI-4-488-577

405 / 488 / 577 / 640

Performance by Wavelength

Wavelength

Optical Power Min (1)

CW Stability (typ.) (2)

Max Rise Time (typ.) (3)

Max On/Off Frequency (4)

405

Optical Power Min (1) 300 mW

CW Stability (typ.) (2) < 2%

Max Rise Time (typ.) (3) < 10 µs

Max On/Off Frequency (4) > 1000 Hz

470 or 488

Optical Power Min (1) 1000 mW

CW Stability (typ.) (2) < 2%

Max Rise Time (typ.) (3) < 10 µs

Max On/Off Frequency (4) > 1000 Hz

555 or 577

Optical Power Min (1) 1000 or 700 mW

CW Stability (typ.) (2) < 2%

Max Rise Time (typ.) (3) < 2 ms

Max On/Off Frequency (4) 100 Hz

640

Optical Power Min (1) 400 mW

CW Stability (typ.) (2) < 2%

Max Rise Time (typ.) (3) < 10 µs

Max On/Off Frequency (4) > 1000 Hz

(1) Optical power measured at 100% intensity, 23°C ± 2°C.

(2) Continuous Wave Stability: Standard Deviation relative to mean power, at 100% intensity, 23°C ± 2°C.

(3) Optical power measured at 100% intensity, 23°C ± 2°C.

(4) Measured at 100% intensity, 50% duty cycle

Optical characteristics

Output Power

Up to 1000 mW per line

Optical Stability

Feedback-controlled
< 2% CW stability

Intensity Control

1% steps
100:1 linear dynamic range

Laser Lines

Up to 7 lines : 405 / 445 / 470 or 488
520 / 555 or 577 / 640 / 730 nm

NIR Channel (730 nm)

✔ Deep-tissue imaging
Extended spectral range beyond visible

Beam Quality

Standard multimode output

Fiber Output

400 µm bifurcated

User Alignment

Not required

LDI-4 Series Laser Diode Illuminator General specifications

Output Options

Optical fiber
(400 µm bifurcated, 0.39 NA)

Control Options

TTL (> 2.3 V) | Analog (0–5 V)
USB–DSP (virtual COM port)

Safety

Interlocked housing | Key interlock
IEC 60825 compliant

QUAREP-LiMi WG1

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Dimensions

318 × 234 × 146 mm
(12.5" × 9.2" × 5.75")

Weight

~ 4 kg (~9 lbs)

Operating Temp.

15 to 30 °C

Storage Temp.

-18 to 50 °C

Humidity

< 80% non-condensing

Voltage

90–220 V AC, 50–60 Hz

Warranty

2 years

Available options

Fiber sets

LDI Breakout Box

Fiber Bend Box

External Despeckler

Applications / Techniques

Spinning Disk Microscopy

Widefield Microscopy

Light Sheet Microscopy

Structured Illumination Microscopy (SIM)

Super Resolution Imaging

TIRF

PALM / STORM / DNA-PAINT

FRAP

Photoactivation / Photoconversion

Photoablation

Optogenetics

Neuroscience / Physiology

Cellular Biology

Single Molecule FISH (smFISH)

Spatial Transcriptomics / Proteomics

Digital Pathology